KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.047 | - |
2-methyl-cis,cis-muconate | - |
Rhodococcus erythropolis | |
0.052 | - |
3-fluoro-cis,cis-muconate | - |
Rhodococcus erythropolis | |
0.066 | - |
3-methyl-cis,cis-muconate | - |
Rhodococcus erythropolis | |
0.119 | - |
2,4-dichloro-cis,cis-muconate | - |
Rhodococcus erythropolis | |
0.173 | - |
3-chloro-cis,cis-muconate | - |
Rhodococcus erythropolis | |
0.312 | - |
2-chloro-cis,cis-muconate | - |
Rhodococcus erythropolis | |
6.997 | - |
cis,cis-muconate | - |
Rhodococcus erythropolis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
230000 | 250000 | gel filtration, nondenaturing electrophoresis | Rhodococcus erythropolis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus erythropolis | - |
1CP | - |
Rhodococcus erythropolis 1CP | - |
1CP | - |
Purification (Comment) | Organism |
---|---|
- |
Rhodococcus erythropolis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:4-chlorophenol-grown cell | - |
Rhodococcus erythropolis | - |
culture condition:benzoate-grown cell | - |
Rhodococcus erythropolis | - |
Storage Stability | Organism |
---|---|
4°C, enzyme concentration 0.45 mg/ml, 50 mM Tris-HCl buffer, pH 7.5, 2 mM MnSO4, 0.2 M NaCl, 50% loss of activity after 3.5 months | Rhodococcus erythropolis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(+)-5-Chloromuconolactone | elimination of chloride is not catalyzed | Rhodococcus erythropolis | trans-4-Carboxymethylenebut-2-en-4-olide + Cl- | - |
? | |
2,4-dichloro-cis,cis-muconate | - |
Rhodococcus erythropolis | ? | - |
? | |
2-Chloro-cis,cis-muconate | r | Rhodococcus erythropolis | 5-Chloromuconolactone | the enzyme is unable to catalyze dehalogenation | ? | |
2-Methyl-cis,cis-muconate | - |
Rhodococcus erythropolis | ? | - |
? | |
3-Chloro-cis,cis-muconate | - |
Rhodococcus erythropolis | cis-4-Carboxymethylenebut-2-en-4-olide | - |
? | |
3-Fluoro-cis,cis-muconate | - |
Rhodococcus erythropolis | ? | - |
? | |
3-Methyl-cis,cis-muconate | - |
Rhodococcus erythropolis | ? | - |
? | |
cis,cis-Muconate | - |
Rhodococcus erythropolis | ? | - |
? | |
additional information | completely inactive with 2-fluoro-cis,cis-muconate | Rhodococcus erythropolis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000-42000, SDS-PAGE | Rhodococcus erythropolis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0567 | - |
2-methyl-cis,cis-muconate | - |
Rhodococcus erythropolis | |
0.8 | - |
3-methyl-cis,cis-muconate | - |
Rhodococcus erythropolis | |
2.2 | - |
2-chloro-cis,cis-muconate | - |
Rhodococcus erythropolis | |
4.33 | - |
cis,cis-muconate | - |
Rhodococcus erythropolis | |
6.07 | - |
3-fluoro-cis,cis-muconate | - |
Rhodococcus erythropolis | |
22.3 | - |
2,4-dichloro-cis,cis-muconate | - |
Rhodococcus erythropolis | |
60.7 | - |
3-chloro-cis,cis-muconate | - |
Rhodococcus erythropolis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Rhodococcus erythropolis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.2 | 8.2 | 4 C, 36 h, 40 mM Tris-HCl, MOPS or HEPES buffer, enzyme concentration 0.03 mg/ml | Rhodococcus erythropolis |